An improved zinc-finger nuclease architecture for highly specific genome editing. Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, …

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases. Lim K, Cho SI, Kim JS Nat Commun. 2022 Jan 18;13(1):366. doi: 10.1038/s41467-022-27962-0. PubMed Article Plasmids from Article. ID

a ZFD (zinc finger deaminase) architecture. Split-DddA tox halves are fused to the C terminus of ZFPs (zinc finger proteins) (C type). b Optimization of the ZFD platform using pTarget libraries ...

Addgene suggests sequence verifying the construct before starting your experiments. Bacterial cell-based two-hybrid (B2H) reporter vector. ... Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, Eichtinger M, Fu F, Porteus …

Zinc finger proteins are transcription factors with the finger domain, which plays a significant role in gene regulation. As the largest family of transcription factors in …

Zinc Finger MYND-Type Containing 8 (ZMYND8) is epigenetically regulated in mutant Isocitrate Dehydrogenase 1 (IDH1) glioma to promote radioresistance. Carney SV, Banerjee K, Mujeeb A, Zhu B, Haase S, Varela ML, Kadiyala P, Tronrud CE, Zhu Z, Mukherji D, Gorla P, Sun Y, Tagett R, Nunez FJ, Luo M, Luo W, Ljungman M, Liu Y, Xia Z, Schwendeman …

Depositing Labs: Scot Wolfe, Nathan Lawson. This plasmid kit contains reagents for engineering zinc finger arrays by modular assembly method. This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered from ...

A Better Way toShare Science. A Better Way to. Share Science. Type 3 or more characters for results. We distribute 141,398 plasmids on behalf of 5,950 labs from around the world. Our collection also contains 877 ready-to-use viral vectors and 188 recombinant antibodies. These materials have been requested 2,056,313 times and shipped to 111 ...

Plasmid pSG5-FLAG-mEKLF Zinc Fingers from Dr. James Bieker's lab contains the insert EKLF and is published in Mol Cell Biol. 2001 May;21(9):3118-25. This plasmid is available through Addgene.

Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab. ... Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer.

Encodes ZFHD1, a member of the zinc finger homeodomain transcriptional factor family. Binds to the 62 bp promoter region of ERD1 (early responsive to dehydration stress 1). Expression of ZFHD1 is induced by drought, high salinity and abscisic acid. NEW. Try the new Gene table. Try the new Transcript table.

Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene …

DNA binding site to CCR5 left-handed zinc finger (GTCATCCTCATC) inserted upstream of the CAG promoter. Vector type. Mammalian Expression Growth in Bacteria ... Principal Investigator, cite the article in …

Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, …

Addgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues. Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, …

Engineered zinc finger nucleases can stimulate gene targeting at specific genomic loci in insect, plant and human cells. ... strain available through Addgene as part of the Zinc Finger Consortium ...

Plasmid pX330A-1x2 from Dr. Takashi Yamamoto's lab contains the insert humanized S. pyogenes Cas9 nuclease and is published in Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. This plasmid is available through Addgene.

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Targeted mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE, Maeder ML, Pearlberg J, Joung JK, Peterson RT, Yeh JR Nat Protoc. 2009. 4(12):1855-67. PubMed Article Plasmids from Article ...

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Compact zinc finger base editors that edit mitochondrial or nuclear DNA in vitro and in vivo. Willis JCW, Silva-Pinheiro P, Widdup L, Minczuk M, Liu DR

Kit #1000000005 includes 141 zinc finger plasmids encoding different zinc finger modules from the Sangamo, Barbas, and Toolgen module sets, and can be used to efficiently assemble zinc finger arrays via modular assembly. Also included in this kit are 5 plasmids and a strain for the bacterial two-hybrid (B2H) system used to screen zinc finger ...

Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells. Liu Y, Zhao N, Kanemaki MT, Yamamoto Y, Sadamura Y, Ito Y, Tokunaga M, Stasevich TJ, Kimura H. Genes Cells. 2021 Nov;26(11):905-926. doi: 10.1111/gtc.12893.

Chloramphenicol. Tetracycline. Ampicillin. Ampicillin. Instructions: To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal.

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zinc Finger Consortium Expression Vector Kit v1.0 Unpublished. Plasmids from Article. No plasmids are currently publicly available from Addgene for this article.

Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene …

Zinc-finger domains designed for the loxF8 flanking sequences were evolved based on the established substrate-linked directed evolution of recombinases 2,4,6,7,8. SLiDE links excision activity of ...

In this study, we fused zinc-finger DNA-binding domains (ZFDs) to Cre-type recombinases. We performed systematic analyses to define the optimal spacing and …

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... An improved zinc-finger nuclease architecture for highly specific genome editing. Miller JC, Holmes MC, Wang J, Guschin DY, Lee YL, Rupniewski I, Beausejour CM, Waite AJ, Wang NS, Kim KA, Gregory PD, Pabo …

Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. ... Zinc Finger Consortium Modular Assembly Kit v1.0B Unpublished. Plasmids from Article. No plasmids are currently publicly available from Addgene for this article.

With the genome engineering revolution came epigenome-engineering tools - zinc finger nucleases and TALENs fused to epigenetic modifiers enabled epigenetic modifications at a user-specified locus. Researchers showed …

Programmable nucleases, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and CRISPR systems, have enabled rapid advances in the …